|
KMID : 0384119990190010131
|
|
Korean Journal of Clinical Pathology
1999 Volume.19 No. 1 p.131 ~ p.136
|
|
|
The Change of Mitotic Index in Bone Marrow Chromosome Analysis According to Time in Culture and Use of Giant Cell Tumor-Conditioned Medium
|
|
서헌석:Suh Hun-Suk
김상경:Kim Sang-Gyung/전창호:Jeon Chang-Ho/:Hana Aviv
|
|
|
Abstract
|
|
|
-Abstract-
Background : One of major challenge of cytogenetics is to obtain qualitative
metaphases to achieve a meaningful analysis. Methotrexate (MTX) synchronization and
the Giant Cell Tumor-Conditioned Medium (GCT-CM) have been used t6 improve the
metaphase preparation from hematopoietic malignancies. The purpose of this study is to
determine Mitotic Index (MI) of bone marrow samples under several culture conditions
that may improve the quality of chromosome preparation.
Methods : Sixty nine bone marrow samples were cultured into 3 groups by traditional
methods. The first group was tested for the effect of cell concentration on MI. The
second, for the effect of MTX concentration on MI. The third group was classified into
4 subgroups as follows: 1) MTX only in 24 hour culture 2) MTX and GCT-CM in 24
hour culture 3) 48 hour culture without MTX 4) 48 hour culture and GCT-CM. MI was
calculated as the ratio of metaphase to interphase cells in 1000 cells. Quality of
metaphase was evaluated by classified the metaphase cell into 3 types.
Results : The first and second groups revealed no relationship between cell
concentration, amount of MTX and MI, respectively. The third growed showed
significant differences among four subgroups. The MI increased in subgroups using
GCT-CM and in the 48 hour culture, with greatest increase in group using 48 hour
culture and GCT-CM simultaneously.
Conclusions : The use of GCT-CM medium and prolonged culture improved the
quality of metaphase cells and MI. It is therefore beneficial to use these conditions in
cytogenetic studies on bone marrow in hematologic disease.
|
|
|
KEYWORD
|
|
|
Cytogenetic stud, MI, CGT-CM,
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|